small group tours austria

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Tours to Austria

Austria boasts natural beauty that's simply breathtaking. Have your own Sound of Music moment atop an Alpine mountain and be wowed by the charming lakeside villages on an Austria tour. And don't forget the mouth-watering food and drink. 

Ways to explore Austria

Rail journeys, discover our austria tours.

Discover verdant mountains, glassy lakes, towering pine trees and colorful wildflowers on our  small group  Austria tours. 

Escape the daily grind and don your walking boots to explore the breathtaking scenery of the Alpine region with our  Austria walking tours . Trek through lush forests, colorful meadows, and ascend the higher ridges by cable car for panoramic views of the valley. Dip tired feet into the refreshing waters of Krottensee Lake and savor local apple strudel and Zirbenschnaps along the way.

Ready to book an Austria tour but new to Explore? We've answered some of the  most-asked questions  about small group trips.

Our most popular Austria tours

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London to Istanbul Rail Adventure

London to Istanbul Rail Adventure + Cappadocia Extension

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Unforgettable experiences.

From big wows to hidden gems, our tours leave you feeling that you've really explored.

Expert tour leaders

Chosen for their great knowledge of your destination and a passion to make your trip extraordinary.

Small groups

Average groups of 11; solos, couples and friends, united by a desire for authentic experiences.

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How we operate sets us apart; our flexible booking policy, our loyalty scheme & sustainable approach.

Austria tour reviews

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Need some inspiration for your Austria tour?

7 questions answered for your first small-group adventure tour

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• Small Group Tours

Showing  14 Trips in Austria

Best of Central Europe

• Brochure Price: USD 3,775
• Special Deal 30% OFF - USD 1,133
• Total Price from: USD 2,642

Journey through Central Europe & Romania

• Brochure Price: USD 6,265
• Special Deal 30% OFF - USD 1,879
• Total Price from: USD 4,386

Vienna to Dubrovnik

• Brochure Price: USD 7,120
• Special Deal 20% OFF - USD 1,424
• Total Price from: USD 5,696

Cycle the Danube

• Brochure Price: USD 2,280
• Special Deal 20% OFF - USD 456
• Total Price from: USD 1,824

Grand Odyssey: Enchanting Balkans & Central Europe

Essence of Austria

• Brochure Price: USD 3,479
• Special Deal 4% OFF - USD 150
• Total Price from: USD 3,329

Vienna to Tirana Tour: Central Europe & the Balkans

Athens to Vienna Tour – Balkans & Central Europe

Christmas in Central Europe, Vienna to Berlin

Christmas in Central Europe, Berlin to Vienna

Cycling Vienna to Budapest

Cycling Prague to Vienna

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Austria Small Group Trips

Looking for a small group tour to Austria? We currently offer 10+ small group tours in Austria having received many customer reviews. Our tours have been handpicked to provide you with the finest selection of small group tours that Austria has to offer. If you are a solo traveler or a couple looking to meet other people, joining a small group tour is a great way to meet other like-minded travelers. Bon voyage.

Austria Small group Tour Reviews

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Vienna à la carte

Your specialist for private & small-group tours in vienna, featured tours, latest reviews.

Robert was an excellent guide, very knowledgeable- not just about the major sites but also the wider European history of the region. Driving was excellent and thoroughly enjoyed our day. Highly recommend.

This was one of our highlights during the trip, breath-taking views and clean crisp air coupled with the charm of Salzburg at Christmastime - made the long trip worthwhile. Rui was a great guide (and driver) and extremely knowledgeable - he also drove us to our hotel in the end. I recommend this tour to anyone visiting Vienna - it is amazing!

Fantastic day out with our guide Rui. Despite the snow and inclement weather he made it fun and showed us interesting spots on the way to Salzburg. Enough time in Salzburg for a personalised tour and an independent roam around. An excellent and safe driver with a very comfy mini bus. Would highly recommend.

Our guide was very knowledgeable and helpful. I was with my Mom & sister and he was very accommodating since my Mom was in a mobilty scooter. The stops along the way were beautiful and he found us nice halfway points for a quick break.

I went with my rather mature 11 year old son, and we found this tour to be very good. Our tour guide was wonderful, compassionate, and informative. While I understand this tour is not appropriate for younger kids, it was appropriate for my child and important history for us to learn.

It was a nice group of people. Robert was a great guide & driver. Despite the cold, rainy day, it was a fun trip!

Exceptional. Great driver. Savvy and friendly local guide. Listen to their recommendations for restaurants!

Our guide, Joseph, was amazing, knowledgeable, patient, kind and understanding of the different needs of our group and very helpful! Loved the picture taking, the history lessons and our special tour stops! Thank you, Joseph!

It was a long time in the car but the time in Salzburg was wonderful and the drive thru the alpine lake region was beautiful

Excellent trip great tour guide and bus driver. Very professionals and polite. The tour was very interesting and impressive. Something very amazing to never let us forget about the terrible things that happen there. If you have the opportunity this is your sign for it!

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Austria and Germany Small Group Tours

28 austria and germany small group tours with 18 reviews.

Hiking & Trekking

Individuelle Alpenüberquerung auf dem E5 - Die klassische Hüttentour von Oberstdorf nach Meran

Explorer Active Self Drive

Munich, Austrian Alps, Dolomites in a Porsche: Pre-set sat-nav guided

"We navigated through great scenic points and great restaurants. Definitely a great memory of a lifetime!" BarryHung, traveled in September 2019

Individuelle Alpenüberquerung von Garmisch nach Sterzing - Von Hotel zu Hotel über die Alpen

In-depth Cultural Christmas & New Year +1

Europe Christmas Markets: Munich to Budapest

"Great markets, wonderful food and drink, beautiful city. We ended in Budapest where the rain drove me into the thermal baths for the day." Marilyn, traveled in December 2017

Active Explorer Ski, Snowboard & Snow +1

Three Country Ski Tour

Family Mountain Hikes Self Guided Walking Holidays Explorer +2

Trans Tyrol - Garmisch to Innsbruck

"The briefing and guide notes were excellent and the route was great." World, traveled in June 2011

In-depth Cultural Family Self Drive +1

Romantic Germany Self Drive

Bicycle Family

Highlights of Lake Constance by Bike

Explorer Family Christmas & New Year +1

Germany and Austria in 8 days - Covering Munich Salzburg and Vienna

Bicycle Family Self-Guided Cycling +1

Cycling on the Danube from Passau to Vienna

"Cycling through the Wachau wine growing area. Lovely scenery and saw some of the grape harvest!" Jane, traveled in January 1970

Family Explorer

Munich to Salzburg Cycle

Hiking & Trekking Active Mountain Hikes +1

Walking Bavaria Tyrol around Mount Zugspitze

Explorer Family

MUNICH - INNSBRUCK - SALZBURG - HALLSTATT - VIENNA

Panoramic Alps Cycle Tour from Innsbruck to Salzburg

Festival & Events

OPERA AT THE OPEN STAGE IN BREGENZ, AUSTRIA: Der Freischutz

Reviews of small group tours to austria and germany.

With this guided tour, we felt like we were locals. We navigated through great scenic points and great restaurants. Definitely a great memory of a lifetime!

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Destination: Austria

Austria tours for seniors

Odyssey offers easy, convenient, and relaxed escorted small group tours across Western Europe and beyond. We explore Austria’s fairy-tale natural beauty, its storied imperial heritage, World Heritage Sites, and incredibly beautiful cities, with some truly spectacular scenery along the way. This and more is all waiting to be explored on one of Odyssey’s small group tours of Austria, designed for the senior traveller, and led by experienced, and enthusiastic like minded people.

Continues below

Austria Tours

Hand crafted tours for mature World Travellers

Habsburg Art and Classical Music small group Tour

Visiting Austria, Czech Republic

From the mediaeval jewel of Cesky Krumlow to the grandeur of the Habsburg’s summer palace in Vienna , our small group will embark on a 21 night journey to learn about these vibrant and artistically rich cities . We delve into the history of the famous artists, musicians, writers, architects and composers from Austria , Hungary , Poland and the Czech Republic.

From A$15,245 AUD

Grand tour of Europe

Visiting Austria, Belgium, Croatia

For the senior couple or mature solo traveler seeking a small group educational tour focussed on exploring and learning with like minded people in Europe .

From A$47,275 AUD

With breathtaking mountain scenery and striking baroque architecture, Austria is a country of majesty. Here, the great outdoors beckon and it is easy to get lost in the gorgeous alpine landscapes framed by bright blue lakes and verdant green valleys, but the country’s sophisticated cities also entice travellers with imperial castles and palaces, fascinating museums and a vibrant restaurant scene. Whether you’re captivated by the compelling history of the Habsburg family and the Austro-Hungarian Empire, yearning to explore the towering Bavarian alps or eager to try authentic Austrian cuisine, you will find what you’re looking in this Central European beauty. Let Odyssey Traveller take you on a journey you will never forget.

Marvel at the grandeur of Vienna with its dazzling opera houses, monumental Ringstrasse palaces and elegant coffee houses. Try the best of Austrian cuisine by digging into Tafelspitz or goulash in one of the city’s rustic restaurants, explore the many museums or stroll through the compact city centre. Salzburg is the stuff of fairy tales with its old town tucked away in the hills, ornate architecture, beautiful parks and history of classical music.

Outside of the cities, you can ski, hike or bike your way through snow-tipped mountains and deep valleys, explore quaint villages along the River Danube and swim in some of the world’s most beautiful alpine lakes.

Articles about Austria

Questions About Austria

Questions about Austria for mature and senior travellers seeking a small group tour in Europe as a couple or solo traveller . Tour director and local guide provide day tours to share knowledge about Vienna and Austria.

Music and the Austrian Habsburgs: The Definitive Guide

Music and the Austrian Habsburgs Members of the Habsburg royal family circa 1764. Francis I, Holy Roman Emperor (r. 1745-1765), Maria Theresa, Holy Roman Empress (r. 1740-1780), and their eleven children. Their daughters Marie Antoinette…

Schönbrunn Palace Austria

Schonbrunn Palace, also referred to as Schoenbrunn Palace, is Vienna's most popular attraction and it is not hard to see why. The opulent summer palace of the Habsburgs is a UNESCO World Heritage Site and is considered to be one of the most important cultural, architectural and historical sites in Austria. The palace complex features 1441 rooms (40 of which are open to the public), magnificent gardens, a maze, a palm house, the oldest zoo in Europe and an outdoor swimming pool.

Belvedere Palace Austria

Belvedere Palace (Schloss Belvedere) is a masterpiece of Baroque architecture and as much as piece of art as the impressive works housed in its interior. The large complex actually features two palaces (Upper and Lower) and beautiful grounds. Originally built as a summer residence for Prince Eugene of Savoy, the galleries of the Upper Belvedere focus on Austrian art from medieval times to present day, emphasising Austrian culture and history while the Lower Belvedere hosts temporary exhibitions. The grounds also feature the Orangery, Palace Stables which have been converted into a medieval art exhibition and magnificent gardens. The famous historical palace complex is a UNESCO World Heritage Site and a short drive from the centre of Vienna.

Melk Abbey Austria

A vivid yellow Baroque jewel, Melk Abbey, or Stift Melk, is the most famous abbey in Austria

Eggenberg Palace Austria

Eggenenberg palace, a world heritage site. This baroque palace is considered to be a national treasure in Austria.

Touring Austria

The need to know

Getting around

Odyssey travels by coach and occasionally uses local transport, including trains and ferries. Specifics are always outlined in your tour itinerary. Trains, operated by the Austrian Federal Railways, are a fast and efficient option to travel around the country. Buses can be handy in getting you to remote regions and villages, and bus services operate in most cities. Domestic flights are available, though can be a costly option.

Accommodation

In major cities in Austria, Odyssey stays in centrally located 3-4 star hotels, with easy access to public transport. In smaller towns or rural areas, we usually stay in family-run hotels or guesthouses. On our long stay tours, during which you spend the length of the tour in a single location, we use serviced apartments.

Tour Guides

Odyssey always engages local guides with regional knowledge to ensure an authentic experience during which you can learn as much as possible about the history and culture of places you visit.

Geography, environment and weather

Austria is a landlocked country, covering 84k square kilometres. The terrain in Austria is highly mountainous with only 32% of the country below 500m. Just over 40% of Austria is covered by forest, while one-fifth of the country is pasture and meadow located at various altitudes.

Austria has a predominantly alpine climate, with cold winters and warm summers. If travelling to Austria in the winter months, be sure to pack some warm clothing.

World Heritage sites

Austria has 10 UNESCO World Heritage Sites. You can view the official list of the sites here (https://whc.unesco.org/en/statesparties/at). While every site is worth a look, here are a few highlights:

• Wachau cultural landscape, a stretch of the Danube Valley which preserves many architectural and agricultural traces of its historic evolution
• The Semmering Railway, which spans over 41km of high mountains, and stands as a stunning achievement of civil engineering
• Beech Forests of the Carpathians

Festivals and events

With its storied history and famous cultural institutions, Austria has many festivals and events each and every year.

The Viennale international film festival has been running annually since 1960, and attacts filmmakers and film buffs from all over the world. Mörbisch Operetta Festival is a festival dedicated to hosting operattas, and takes place at the visually-stunning locale of Lake Neusiedl.

The International Art Fair is hosted annually in Vienna every autumn, and features a dazzling array of artworks and installations by internationally-renowned artists.

Reading list

• Walking in Austria, by Kev Reynolds
• The Austrians: A Thousand Year Odyssey, by Gordon Brook-Shepard
• The World of Yesterday, by Stefan Zweig

Eating and Drinking

Wienerschnitzel is the nation’s most famous culinary export, with each region having their own version on this ubiquitous Austrian dish. Numerous other Austrian dishes centre around meat, including Tafelspitz, Vienna Wurst, and Austrian Goulash, the local take on the famous Central European meat stew. Other savoury offerings include Erdäpfelsalat (potato salad) and spargel (spring asparagus). If you’re in the mood for something sweeter, there is no shortage of mouth-watering deserts, including apple strudels, kaiserschmarrn and sachertorte (Viennese chocolate cake with apricot jam).

Austria is credited with introducing coffee to Europe in the form of its coffee houses, and coffee remains an integral part of Austrian social life. Beer is popularly consumed throughout the country, while schnapps are sometimes served at the end of a meal.

Health and Safety

Generally Austria is safe to travel around in, though always exercise common sense while travelling.

Electrical supply

Whenever you travel overseas, it’s always wise to take an appropriate travel adapter. Austria operates on a 230 supply voltage and 50Hz. Austria uses both the Type C and Type F plug, so be sure you have an adapter handy as needed.

The best of Austria

Schönbrunn Palace

Schönbrunn Palace, the glittering former residence of Austria's Hapsburg rulers

Belvedere Palace

The complex at Belvedere Palace actually features two impressive Baroque palaces that have been converted into celebrated art galleries, featuring work by some of Austria's best known artists.

Schloss Eggenberg

Schloss Eggenberg, the ornate and eye-catching castle near the city of Graz. Eggenberg Palace in Graz is the most significant Baroque palace complex in Styria.

Tour Reviews

I was impressed by the number of places we visited. It was a very full itinerary which I like. We saw things of interest every day. I see no fault with the tour.

Participant 2017

Anglo French Tour | Normandy, Brittany, Channel Islands tour

Our guide was a great ambassador for his country, wide knowledge of literature, history, customs, industry, fishing - also a perceptive and sensitive person who inter-acted so willingly with all of us!

Participant 2016

Iceland cultural and wilderness small group tour

The country was apparently calm and safe. People were friendly and helpful. Such a pity there has been such a down turn in tourism. Ros & Tony S. Sep '17

Small group tour of Ancient Turkey

The geographical wonders offered by Iceland and Greenland left me in awe. The grandeur and spectacle of the countryside is breathtaking. Our leader (Rob) was excellent: well-researched, friendly, organized and good-humoured.

It is hard to believe that such a small country can contain so much dramatic scenery. Icecaps and glaciers, huge waterfalls, volcanos and hot bubbling mud, and those wonderful fjords and high mountain passes

Every day was different with so much to see and do. Volcanoes, craters (including the pseudo ones), bubbling mud pools, and the active Strokkur Geyser, the bird life, black sand beaches, and those beautiful Icelandic horses. The time flew by...

We felt safe in Turkey and found the people friendly and helpful. Prices were excellent and the food almost universally magnificent. There is so much to see in the natural and built environment and the wonderful archaeological sites. It is sad to see the ordinary people and businesses suffering from the lack of tourists. Mary B. Sep '17

Our guide Filiz was wonderful, very well informed, historical insights very helpful, her care for our well being exceptional. Could not have wished for a better tour leader.

What time zone is Austria in?

Austria has a single time zone, Central European Standard Time (UTC+1). Austria observes daylight saving time from the last Sunday in March to the last Sunday in October.

Is tipping customary in Austria?

If you’re on an Odyssey tour, we take care of tipping so you don’t need to give it a second thought. However, in your free time, or if travelling independently, it’s essential that you tip an appropriate amount for services. Tipping is fairly common in Austria, with some restaurants and hotels including a service charge with the bill. Tipping of taxi drivers is also common, typically done at 10% of the fare.

What is internet access like in Austria?

Internet access is easily accessible, and most hotels, cafes and bars will offer it.

Can I use my mobile phone in Austria?

Check with your cell phone provider to see whether you’re able to make calls and use data while in Austria. Many providers will allow you to pay a daily fee that allows you to make calls and check the internet while only being charged your regular rates. However, be certain to inform your provider that you’re heading overseas, because just like a bank they can turn off your service as a result of unusual activity.

Which are the best cultural experiences in Austria?

Austria offers a wide range of cultural experiences. Museums, architecture, music and art – Austra is not short of cultural history and events.

Salzburg – the birthplace of Mozart, full of churches, castles and palaces, with an old town now a UNESCO world heritage site.

Vienna – Austria’s capital is famous for its cultural events, imperial traditions and modern architecture.

Christmas markets – from the middle of November until the end of the year – do not miss the magical Christmas markets, full of seasonal treats.

Museum of fine arts, Vienna – the Museum of fine Arts in Vienna has an unrivalled collection of old masters, including a vast selection of works by Pieter Bruegel the Elder. You will also find Ancient Egyptian and Greek and Roman Antiquities and Medieval Art housed in this grand Ringstraße building.

Schönbrunn Palace, Vienna – The Habsburgs’ summer residence is Austria’s most popular tourist attraction. Take a guided tour through the Imperial ceremonial rooms or stroll through the freely accessible gardens.

The Vienna boys choir – The Vienna Boys Choir is one of the most iconic cultural institutions in the country. A foundation charter issued by Emperor Maximilian I in 1498. Ever since, the Vienna Boys’ Choir has been a fixture in Austrian musical life.

Articles relating to Austria published by Odyssey Traveller

• Music and the Austrian Habsburgs
• Eight Amazing Rail Journeys
• History and European Pilgrim Walks

For all the articles Odyssey Traveller has published for mature aged and senior travellers, click through on this link .

External articles to assist you on your visit to Austria

• The History of Opera in Vienna
• Austrian weather
• Austrian Food Culture

Responsible travel tips for Austria

• Learn at least the local greetings to break the ice. Although many locals speak English, the more you know of the native language, the greater your experience of the country will be.
• Carry a business card in your wallet or purse from your local hotel, to assist you with the return journey if you do become lost.
• Always ensure that you are covered by travel insurance. If you need advice on this feel free to contact Odyssey and we’ll be able to help.
• When travelling independently, make sure you check the opening hours of shops and museums so that you don’t miss out! Museums and galleries are often closed on Mondays. Also be certain to check whether your trip coincides with any public holidays, so you can plan accordingly.
• Consider contacting your bank to inform them that you may be making purchases overseas. Otherwise, they may flag any activity on your account as suspicious. Also, check which ATMs and banks are compatible with your cards, to ensure you can withdraw cash with minimal fees.
• Before departing, make sure you have a number of euros in a range of denominations. You don’t want to be carrying around enormous amounts of cash, but take enough to make it easy to pay in locations that might not accept credit card. It will also help you avoid card transaction fees, and it makes tipping a breeze.

About Odyssey Traveller

We specialise in educational small group tours for seniors, typically groups between six to 12 people from Australia, New Zealand, USA, Canada and Britain. Our maximum number of people on a tour is 18 mature aged travellers.We have some 150 tours and offer 300 scheduled departures on offer each year. Odyssey has been offering this style of adventure and educational programs since 1983.

Odyssey Traveller is committed to charitable activities that support the environment and cultural development of Australian and New Zealand communities.

Odyssey Traveller scholarship for Australia & New Zealand University students.

We are also pleased to announce that since 2012, Odyssey has been awarding $10,000 Equity & Merit Cash Scholarships each year. We award scholarships on the basis of academic performance and demonstrated financial need. We award at least one scholarship per year. We’re supported through our educational travel programs, and your participation helps Odyssey achieve its goals. Students can apply for the scholarship by clicking on this link to find out more details.

Join our loyalty program when you join an international small group tour.

Every International small group tour taken typically contributes to your membership level in our Loyalty Program for regular travellers. Membership of the alumni starts when you choose to take your first international small group tour with Odyssey Traveller, discounts in tour pricing for direct bookings accrue from your third tour with Odyssey Traveller. To see the discounts and benefits of being a Bronze, Silver, Gold, and Diamond alumni member with us, please see this page .

For more information on Odyssey Traveller and our educational small group tours, visit and explore our website. , remember to visit these pages in particular

• Terms and conditions applicable for booking an Odyssey Traveller tour.
• FAQ’s about Odyssey Traveller

Alternatively, please call or send an email.

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*Prices below are per person based on double occupancy and include airline taxes, surcharges and fees of $0, which are subject to change until final payment is made. All tour prices (excluding airline taxes, surcharges, and fees) are guaranteed.

Departure Selected

Special event, please note, guest 1 details, guest 2 details, trip details, additional options, guest information.

Alpine Splendor: Switzerland and Austria

The towering peaks of the Alps serve as the stunning backdrop for our Swiss and Austrian exploration.  As our small group discovers mountain villages and European cities, rides the famed Glacier Express , cruises on glassine lakes, and ascends snowcapped summits, borders disappear and the region’s history – and beauty – enchant one and all.

Tour Details

From $6,292

Small Group Highlights

• Lovely Lucerne, including  Kapellbrücke  covered bridge
• Cogwheel train to Mt. Pilatus
• Famed  Glacier Express  Alpine train route
• Gornergratbahn  railway with views of the Matterhorn
• Private Lake Lugano cruise
• Visit to Bellinzona’s Castelgrande, a UNESCO site
• Alfresco  grotto  lunch
• Tour and tasting at a Swiss winery
• Baroque Old Town of Salzburg
• Eagle’s Nest, a Nazi clifftop retreat
• Medieval Hohensalzburg, one of Europe’s finest castles
• Intimate classical music concert

Day-by-Day Itinerary

The Swiss-Chalet Merlischachen stands just a short drive from Lucerne and directly on the shores of Lake Lucerne, offering guests a view of the water and its mountainous surroundings.  The hotel’s amenities include a traditional Swiss restaurant, lakeside bar and lounge, sauna and spa with massage services (for a fee), laundry and dry-cleaning service (for a fee), and complimentary Wi-Fi internet access.  Guest rooms are located in the Schloss-hotel building of the hotel, which features a rooftop terrace and park on the lake.  All rooms have private bath with hair dryer, lake view, minibar, in-room safe, TV, and phone.  Please note: The hotel does not offer air conditioning in guest rooms.

Tucked into the northwest corner of the walkable mountain town of Zermatt, Resort Hotel Alex offers a comfortable stay with incredible views of the surrounding Alps.  This cozy, lodge-style hotel presents a traditional façade, but offers a wide range of state-of-the-art amenities.  In addition to a variety of dining and drinking options, the Alex offers a large indoor grotto-style swimming pool, full spa and sauna with a range of treatments (some at additional cost), fitness center, tennis and squash courts, outdoor terrace and lounge amid landscaped gardens, laundry and dry-cleaning services (for a fee), and complimentary Wi-Fi internet access.  Each guest room has private bath with hair dryer, in-room safe, TV, and phone. Please note: Due to its elevation and generally cool Alpine climate, this hotel does not offer air-conditioned guest rooms.

Situated on a quiet street in a pedestrian area of Lugano, the Luganodante Hotel sits just a five-minute walk from the town’s namesake lake.  After a 2020 renovation, the hotel now includes a bistro and cocktail bar inspired by famed alchemist Nicolas Flamel, lobby snack corner with tea and coffee, laundry and dry-cleaning service (for a fee), and complimentary Wi-Fi internet access.  Air-conditioned guest rooms include private bath with hair dryer, tea- and coffee-making facilities, minibar with complimentary soft drinks, TV, and phone.

A true Alpine lodge with wonderful views of the town of Seefeld and its surrounding peaks, Hotel Seelos offers modern amenities in a comfortable mountain setting.  The hotel sits just a short walk from Seefeld’s main street, the Olympiastrasse, named in honor of the several Winter Olympics competitions held here.  The hotel has a restaurant and bar, and includes a sauna and complimentary Wi-Fi internet access.  Guest rooms are decorated with Alpine touches and have balconies with views of the Alps, private bath with hair dryer, TV, and phone.  Please note: The hotel does not offer air conditioning in guest rooms.

Imlauer Hotel Pitter Salzburg was extensively renovated in 2014 after being originally opened in 1864.  Located in Salzburg’s New Town, the hotel sits a short walk away from the Mirabell Palace and Garden and a 10-minute walk from the historic Old Town.  Amenities include a beer cellar, a rooftop restaurant and bar, fitness center, sauna, laundry and dry-cleaning services (for a fee), and complimentary Wi-Fi internet service.  Air-conditioned guest rooms have private bath with hair dryer, in-room safe, tea- and coffee-making facilities, TV, and phone.

Guests will have the opportunity to be in Salzburg during this classical music and performing arts festival (no formal activities planned).

Tour Price Includes

• Round-trip air transportation from listed cities
• 12 nights’ accommodations in Superior First Class and First Class hotels
• 22 meals: 12 breakfasts, 3 lunches, 7 dinners
• Extensive sightseeing as described, including all entrance fees
• Services of an Odysseys Unlimited Tour Director
• Private motorcoach transportation throughout your trip
• Luggage handling for one bag per person
• Gratuities for local guides, dining room servers, airport and hotel porters, and all drivers
• $100 frequent traveler credit towards your next Odysseys Unlimited tour ($200 after 3 tours; $300 after 10 tours)

Average Daily High by Month (°F )

Average temperature (°f ), dates & prices.

Airline upgrades (subject to availability; pricing subject to change) on round-trip Trans-Atlantic flight: Business Class – $3,895 per person in 2024; $3,995 per person in 2025. Premium Economy – $1,795 per person all dates

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Post-Tour Extension

Vienna – On Your Own

Savor an unstructured stay in this city of arts and culture. Music, opera, and museums abound.

Post-Tour Extension Price Includes

• Rail transportation Salzburg/Vienna
• 3 nights’ accommodations at Hotel Kaiserhof (First Class)
• 3 meals: 3 breakfasts
• Vienna city orientation tour with local guide
• Transfer from rail station and to airport

Accommodations

The stylish, family-owned Hotel Kaiserhof sits in a convenient location near the center of Vienna, offering a perfect location from which to explore this cultural gem of a city.  The hotel’s history stretches back to its founding in 1896 when it was one of the first hotels in Europe to offer an elevator, central heat, running water, and electricity; it has long been known as one of Vienna’s finest hotels.  Amenities include a bar, fitness center, sauna, steam bath, laundry and dry-cleaning service (for a fee), and complimentary Wi-Fi internet access.  Air-conditioned guest rooms have private bath with hair dryer, tea- and coffee-making facilities, in-room safe, minibar, TV, and phone.

Tour Directors

“Thanks to Max our tour was even better. His knowledge and caring personality made the trip. Keep him on your guide list. He’s the best.”

Lee Ann Rippentrop Minneapolis, MN

“He was the best tour guide we have ever experienced on a trip.”

Lynn & David Hall Tulsa, OK

“Sabine was experienced, knowledgeable, and fun!”

Donna Willson Brewster MA

“This was our 7 th Odysseys tour and we would rate Gabe at the top of our list.”

Allan Bauer Hackensack, NJ

“She was warm, welcoming, and did everything she could to make everyone’s experience enjoyable. She also tended to the special needs of some of the tour group.”

Anne & Robert Lloyd Westbrook, CT

"She was the best as she seemed to enjoy the daily trip experiences as much as we did. Gave clear information constantly; answered questions thoroughly; helped us all."

David Clark Stillwater, NY

“Chiara pays a great deal of attention to little things as well as big. I have been a tour guide in both Europe and the U.S., and she was just about the best I have ever encountered.”

Barney & Susan Milstein Portland, OR

Odysseys Unlimited Tour Director

“Eva was an outstanding tour director with her sense of humor & caring personality.  She made us feel like part of a family.”

Charlotte Taggart Topeka, KS

"We thoroughly enjoyed Sara as our tour guide. She made us laugh and sing and have a wonderful time. She is so full of life and enthusiasm you couldn't help but be in a great mood and have fun."

Paula Lauer Whitehouse, OH

"Angel was a great tour guide. He was always eager to share his knowledge of Switzerland and Austria with an interesting manner, as well as a great sense of humor.”

Bill & Jan Tecklenburg Broomfield, CO

“Her personality was fun-loving and entertaining, all the while being extremely professional and detail oriented…Outstanding tour director.”

Steven & Marie Olson Normandy Park, WA

“Sonia did an excellent job as our tour guide.  She went beyond the call of duty many times during the trip.”

Edgar & Linda Schmidt Howel, NJ

“Raffe was exceptional! He has great knowledge to share with his group. He is compassionate, caring, exceptionally funny, and full of love for his job…Tour director extraordinaire!”

Shirley Sietsema Merced, CA

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Physical Activity Level

• Walk 2-4 miles per day, often on uneven terrain in natural settings
• High elevations on excursions to Mt. Pilatus (7,000 ft), Gornergrat (10,285 ft), Nordkette (7,401 ft), and Eagle’s Nest (6,017 ft); elevations at hotel locations are not severe (from 1,400 ft to 5,200 ft)
• Several long days of travel using multiple modes of transportation

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Switzerland and Austria

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13 Day Tour to Zurich, Salzburg, Vienna, Innsbruck and Zermatt

13 days, 4 countries and 14 cities

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12 Breakfasts, 5 Dinners

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One of our most popular trips going to Austria and Switzerland, this incredible duo also delivers a visit to the little Liechtenstein capital. The Alps take center stage on this journey, but you’ll also learn about life as a Carinthian nun when you join them for dinner in a former castle.

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13 days itinerary trip from Zürich to Zürich visiting 4 countries and 14 cities

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Sightseeing highlights.

Explore Salzburg and Vienna with a Local Specialist

Discover Liechtenstein, Zürich, Innsbruck and Lucerne

Visit Vaduz, St. Moritz, Stresa, Graz, Lipizzaner Stud farm in Piber and Zermatt

View the Golden Roof and Hofburg Palace in Innsbruck, the Matterhorn and Jungfrau mountains (weather permitting), Chillon Castle, Bern and the Chapel Bridge and Lion Monument in Lucerne

See The Wachau Valley

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Specific transfer information can be found here:

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Breakfast daily and up to half of your evening meals

Must-see sightseeing and surprise extras

Audio headsets for flexible sightseeing

Luxury air-conditioned coach with Wi-Fi in most countries or alternative transportation (such as rail journeys)

Optional Experiences and free time

On occasion, hotels of similar standard and location may be utilized.

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16 Guest Small Group & Private Adventures

Austria & Switzerland Discovery

13 Days Air travel time: 12 Days, 11 Nights Land Journey Begin in Salzburg, the Sound of Music beckons, Vienna, Interlaken, Zermatt, Lausanne & Lake Geneva

12 Days | Vienna, Salzburg, Interlaken, Grindelwald, Zermatt, Lausanne & Lake Geneva

Experience the enchanting melodies of Austria and Switzerland, where the harmonious notes of the Sound of Music blend seamlessly with the velvety allure of Swiss chocolate and the savory richness of Alpine cheese, all against the stunning backdrop of the majestic Alps.

• Vienna: Austro-Hungarian Empire, Music Greats, Opera House, St. Stephan's
• Salzburg: Gardens of Mirabell, Mozart, Dome, Sound of Music
• Interlaken: Lake Thun, Lake Brienz, Bernese Highlands, Alps, Jungfrau, Grindelwald
• Zermatt: Matterhorn, Gornergrat
• Lausanne: Place de la Palud, Hotel de Ville

THIS IS WHY WE SUGGEST JOURNEY FOR YOUR VACATION:

• Expertly Guided: Journey Tour Captain beginning-to-end
• Sightseeing Tours and Admissions, On-Tour Transfers & Luggage Portage included 
• Dining: 15 Meals: 10 Breakfast, 5 Dinners, 1 Aperitive
• European Cuisine with Premium Wine & Cocktails
• Small Group up to 16 Guests
• Luggage Portage throughout your Journey, WiFi
• Meet & Greet at Airport with Included Arrival Transfer
• 11 nights' accommodation at hand-picked Deluxe 4+ to 5-Star Hotels
• 'Master of Your Day' with our Exclusive Premium Sightseeing
• On Tour Transportation: Comfy micro-coach throughout the itinerary
• Customary gratuities excluding the Tour Captain

Journey Quick Itinerary: Hotels

Featuring Unique Experiences with Journey's Slow Travel Concept: Journey's hotels are located in the Historic Centers and locations travelers desire; while on tour include all tourism/city taxes, daily breakfast with table service or buffet (continental in Classic), WiFi, and portage.

• Consider a pre-tour experience in Munich. O PTIONAL BEGINNING: MUNICH , 2 Days/2 Nights EXPERIENCE MUNICH & THE EAGLES' NEST Rich culture thrives in the capital city of Bavaria. TRIP COMMENCES: VIENNA , 2 Days/2 Nights CITY OF MUSIC AND DREAMS. Deluxe: Grand Hotel Wien SALZBURG , 3 Days/3 Nights MOZART, SALT FORTRESS, SOUND OF MUSIC. Deluxe: Sacher Hotel INTERLAKEN , 2 Days/2 Nights HEART OF THE ALPS. Deluxe: Victoria Jungfrau Grand Hotel, Hotel Krebs or Similar ZERMATT , 2 Days/2 Nights LAND OF THE MATTERHORN. Deluxe: Schlosshotel Zermatt, Backstage Boutique Hotel or Similar TRIP FINALE: LAUSANNE , 2 Days/2 Nights LAKE GENEVA. Deluxe: Hotel Beau Rivage Palace, Royal Savoy Lausanne or Similar

Itinerary at a Glance DAY 1-2: VIENNA | DAY 3-5: SALZBURG | DAY 6-7: INTERLAKEN | DAY 8-9: ZERMATT | DAY 10-11: LAUSANNE | DAY 12: DEPART

FIRST THINGS FIRST: Consider our Flight and Early Arrival Options Your flight to Vienna should arrive on Day 1 of the itinerary - this means you should depart your home airport the day prior. Your round-trip travel time on this journey is 12 days (13 days roundtrip with air travel). The trip ends in Lausanne. Airfare is not included. Inquire about our Business Class and Premium Economy Airfare.

Many of our travelers arrive a "full day early" to recover from their overnight flight to Salzburg. Inquire for details.

REMINDER REGARDING THE EURO CURRENCY The currency in Austria is the Euro and the Swiss Franc in Switzerland.  It is always a good idea to have some local currency with you upon arrival.  You can also access more cash with your ATM card.  You may use a credit card in most shops and restaurants.  

Day-by-Day Itinerary

Chapter 1: vienna, day 1: welcome to vienna, austria.

We make our way to Vienna, the City of Music and Dreams, via the scenic road that will allow us to enjoy some of the villages like Hallstatt, St. Wolfgang, St. Gilgen or Mondsee. These are beautiful and historic lakeside villages on our way to Vienna.

Tonight enjoy a dinner in Vienna with your Journey Tour Captain.

About the Cuisine on our Journey:  Family-owned restaurants farm to table Journey’s approach to dining is unique in the travel world. We include Premium Beverages (wine, soda and cocktails) and you choose your dinner from the menu. The Austrian and Swiss chefs and families are proud of their delicious dishes which hail from each of the regions in which Journey travels. And in Salzburg, you will enjoy a variety of dishes with great names like the beloved Schnitzel –  a big, battered, flattened, and fried slab of tender veal served with a lemon wedge and potatoes with parsley, or Strude, which can be cream, custard, ice cream all filled in a variety of pastries (think cherry strudel, plum strudel, etc.), along with Sachertorte (just wait for this one!). While on travels through Austria and Switzerland we will enjoy locally grown produce all served with passion and pride.

Deluxe: Grand Hotel Wien

The Grand Hotel Wien in Vienna is part of the deluxe accommodations offered by Now Journey. This hotel exudes Viennese charm and is ideally located for exploring the city's rich cultural and artistic heritage. Our guests will enjoy delving into the incredible history of the Austro-Hungarian Empire from this luxurious base with daily breakfast included.

BACK TO TOP

DAY 2: EXPLORING VIENNA  

After the adventure, enjoy the balance of the day at your leisure.

Consider a cooking class in Vienna: Make the very best Schnitzel you've ever tasted with this Austrian cooking class. Vegetarians also welcome as we can adapt the menu! $249 per person and lasts about 3 hours.

Journey is Different: Join the Journey Tour Captain tonight for an optional dinner or explore at your leisure.

Chapter 2: Salzburg: The Hills are Alive!  

Day 3: welcome to salzburg.

This morning we make our way to Salzburg, our home for the next 3 days and nights!

Tonight we entertain your palette with cuisine and wines from Austria. Your Journey Tour Captain welcomes you to Salzburg.

Deluxe: Hotel Sacher Salzburg

The Hotel Sacher Salzburg is a luxurious hotel that offers a blend of traditional elegance and modern comfort, making it an ideal choice for travelers seeking a high-end experience. Guests can enjoy a stay of 3 nights with breakfast included. The hotel is perfectly situated for exploring Salzburg, a city known for its baroque architecture and rich cultural heritage. Highlights of the area include the world-famous gardens of Mirabell, Mozart's birthplace, and the Salzburg Dome Cathedral. The "Old Town," a World Heritage Site, is also nearby, offering a wealth of historical and architectural sights. Staying at the Hotel Sacher Salzburg provides a convenient and opulent base for discovering the many attractions of this charming city.

DAY 4: EXPLORING SALZBURG  

Our walking tour includes the world famous gardens of Mirabell, the house where Mozart was raised and the house of his birth along the street Getreidegasse. We enjoy a visit to the Salzburg Dome Cathedral and St. Peter's Monastery.

Our sightseeing includes:

• Gardens of Mirabell
• Mozart's Birthplace House & Residence
• Getreidegass Strausa
• Salzburg Dome Cathedral
• St. Peter's Monastery

We make our way to the impressive Hohensalzburg Fortress that sits atop the city, and time for an optional visit to the Rocky Riding School (Felsenreitschule). This is the theater with a retractable roof and was the stage the Von Trapp Family Singers performed on prior to the escaping the Nazis in The Sound of Music.

Later tonight, join the Tour Captain for an optional dinner or enjoy exploring at your leisure.

DAY 5: SALZBURG AT LEISURE

Enjoy a day at leisure. Consider joining your Tour Captain for an optional Sound of Music biking adventure where you'll visit the iconic filming locations from the beloved movie. You can also immerse yourself in the city's Café Culture by relaxing in one of its charming cafes, such as Café Tomaselli, and savoring a coffee and pastry.

For those who love to shop, a stroll down Getreidegasse, Salzburg's famous shopping street, offers a delightful experience of browsing unique shops and boutiques.

As the day winds down, treat yourself to a Mozart Dinner Concert, where you can enjoy a delightful dinner accompanied by Mozart's music in a historic setting. Whether you're interested in history, music, or simply soaking in the atmosphere, Salzburg has something to offer for everyone.

Inquire about our optional ideas in Salzburg.

DAY 6: TO INTERLAKEN

We make our way to Interlaken today.

INTERLAKEN: HEART OF THE ALPS

Aftewards, enjoy time at leisure until our dinner this evening.

Tonight enjoy a dinner in Interlaken with your Journey Tour Captain.

Deluxe: Hotel Interlaken or Similar

DAY 7: EXPLORING THE ALPS  

Majestic Beauty. On the return Gondola enjoy the sights —  you can also choose to stop half way down and try a ride on a Trottibike back to the village (all downhill) in warmer months. The Trottibike inherited the best features of the scooter and the bicycle. You stand upright on a wide footboard and roll along pleasantly even on bumpy surfaces, thanks to the suspension fork and large tire with tread. Need some speed? The Trottibike awaits you. After all, it has great brakes, so you can stop at any time from Bort to Grindelwald to view the meadows and farmhouses.

Enjoy the balance of your day at leisure.

Journey is Different: Join the Journey Tour Captain tonight for an optional dinner or explore at your leisure.   

DAY 8: ZERMATT & THE MATTERHORN

The town is a car-free zone and has preserved its original character over the years. The region called Matterhorn glacier paradise is Europe's largest and highest summer skiing region. Numerous national ski teams train here during the summer months. The region is legendary amongst mountaineers: the Haute Route, a challenging international route that takes several days to complete, leads from Mont Blanc to Zermatt. Over 248 miles of hiking trails lead through and out of the Matter Valley, including the mule traders' trails, which date back to the 13th century. 

Tonight enjoy a dinner in Zermatt with your Journey Tour Captain.

Deluxe : Schlosshotel Zermatt

DAY 9: ZERMATT  

Enjoy a day at leisure with an optional cogwheel train to Gornergrat (elevation is 10,225 ft.) for fantastic views of the Matterhorn. Or just explore the village and enjoy the ambiance of one of the most beautiful villages in the Alps.

DAY 10: TO LAUSANNE

We make our way to Lake Geneva and our home of Lausanne via the beautful drive. We arrive mid-day, then check in, drop our bags off and enjoy a Discovery Walk.

Tonight enjoy a dinner in Lausanne with your Journey Tour Captain.

Deluxe: Hotel Beau Rivage Palace

The Hotel Beau Rivage Palace, located in Lausanne, Switzerland, is a luxurious five-star hotel renowned for its elegance and exceptional service. Nestled on the shores of Lake Geneva, the hotel offers stunning views of the lake and the Alps. The property boasts beautifully landscaped gardens, a world-class spa, and a variety of dining options, including Michelin-starred restaurants. The rooms and suites are elegantly decorated, combining classic charm with modern amenities to ensure a comfortable and memorable stay. The hotel also features extensive meeting and event facilities, making it a popular choice for both leisure and business travelers. Whether you're looking to relax, explore the scenic surroundings, or indulge in gourmet cuisine, the Hotel Beau Rivage Palace provides an exquisite experience.

DAY 11: LAUSANNE  

Sightseeing includes:

• Place de la Palud  with a visit of thetown hall,  Hôtel de Ville  
• The  cathedral  is recognized as one of the most beautiful in Europe
• You may enjoy an optional visit to the  ancient city ,  the  castle St-Maire  and the " escaliers du Marché " (market's staircases), as well as the  church St-François  which has served 700 years an oasis of serenity. The pedestrian streets and will also help you get to know the atmosphere of Lausanne.

After our experience, the balance of the day is at leisure until our event tonight.

Until our next Journey — Adieu!

Journey is Different: Join the Journey Tour Captain tonight after our Farewell Aperitve for an optional dinner or explore at your leisure for your last night in Switzerland.   

DAY 12: DEPARTURE DAY: Goodbye to Switzerland

We say  Wiadaluaga  (Swiss for Goodbye) today and transfer to the airport or perhaps you extend your stay.

Ready to book this Journey: Click Here

Are you ready for a No Obligation Trip Proposal or Make Your Reservation Request? Click Here  to  Begin

Not quite ready to book the trip chat  to discuss your trip with a journey trip planner:, the austria & switzerland discovery overlander.

ENJOY OUR UNIQUE APPROACH TO TRAVEL We don't do buses. We don't do large groups.

© 2021, 2022, 2023, 2024 NOW JOURNEY LLC

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Switzerland.

Reach new heights

Incredible skiing on the Swiss Alps and delectable Swiss chocolate are only two reasons for visiting Switzerland. Located between Austria, France, Germany and Italy, you can get quite a diverse European cultural experience depending on which region of Switzerland you choose. And though the food, music, art and people themselves are very much influenced by their European neighbors, they are all still uniquely Swiss. You will leave with plenty of memories to savor for a lifetime.

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Destination must-see's.

Matterhorn:

World famous and the symbol for Switzerland, this mountain is the most photographed in the world. It faces the four cardinal directions and is the tenth highest mountain in Switzerland, but because it has a pyramid shape it is very challenging to climb. The first ascent was in 1865 and cost the lives of 4 out of 7 alpinists. It gets its German name from the words matte meaning meadow and horn meaning peak.

This village is like no other. It lies at the foot of what is probably the world’s most famous mountain. It also is located in an enormous hiking and ski region, making it a great vacation spot, even in the summer. This is a car-free zone vacation spot, making it possible to preserve its historical features as well.

As the capital of Switzerland it has managed to retain its historic features. It is a UNESCO World Heritage site. It features 4 miles/6 km of arcades, or as the locals like to call it ‘Lauben,’ making it the longest weather-sheltered shopping in Europe. They also hold the seat of Switzerland’s government, many festivals, a bear park, and beautiful historic buildings that line the streets.

This gateway to central Switzerland sits on Lake Lucerne and has a view of the mountain from wherever you are. Aside from the view it has the Chapel Bridge which is one of Europe’s oldest wooden bridges. A tourist favorite is to visit its souvenir and watch shops, which Switzerland is known for.

Destination Must-Do's

Swiss Chocolate:

It is only expected that the country that created chocolate is the country that consumes the most chocolate as well. It was created by adding milk to chocolate to try to reduce the cost and make it more palatable, which lead to milk chocolate. Switzerland now has over seven major chocolate companies. The best part is if you are a chocolate lover you can tour these companies and try their many different chocolates.

Swiss fondue:

Swiss fondue was created by local villagers with limited access to fresh foods in the winter. They discovered that if you heated the aged cheese with wines, garlic, and herbs then dipped your stale bread it would soften in the flavorful cheese mixture. Families would cook together in one pot and eat by the warm fire, becoming a Swiss winter tradition over time known as fondue.

GoldenPass:

This train ride is like a day vacation. You experience two languages, three regions, and eight lakes. The company focuses on journey and comfort not just transportation. It has seven different lines you can choose from, with the most famous being the panoramic that has panoramic windows that allow you to be one with the landscape. The other is the chocolate train which is a first class trip that lets you experience the production of famous Swiss cheese, the creation of Swiss chocolate, and a chance to see its magnificent castle.

Glacier Express:

This may be the slowest express train in the world, but for a reason. There is so much to experience on this journey that they slow it down for you. It takes you from Zermatt to St. Moritz. The train ride is an eight-hour panoramic journey. You travel into the Alps, go through 91 tunnels, and 291 bridges.

Expert Advice

The United Nations Educational, Scientific, and Cultural Organization encourages the identification, protection, and preservation of cultural and natural heritage around the world. Switzerland has three natural and eight cultural sites that can be visited when in the country.

The currency is the Euro in almost all of Europe. In Switzerland, the Swiss Franc is used (CHF). Your personal bank card may work at local ATM machines in the destination you are traveling to and would dispense local currency. Please contact your bank to determine if this option is available.

You're in for a treat as you truly savor the local flavors of the Alpine region. In Austria, look for the beef soup -- Rindsuppe -- a clear soup with gold color that is as tasty as it looks! The Tafelspitz is beef boiled in broth, often served with an apple and horseradish sauce. It's one of Austria's most savory dishes.

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Switzerland: Hidden Trails & Majestic Peaks

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Discover Switzerland, Austria & Bavaria featuring Oktoberfest

Peaks of Europe: The Alps to The Dolomites featuring France, Switzerland, Liechtenstein, Austria, and Italy

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Magical Rhine and Moselle Rivers

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June 19, 2024

This article has been reviewed according to Science X's editorial process and policies . Editors have highlighted the following attributes while ensuring the content's credibility:

fact-checked

peer-reviewed publication

trusted source

A railroad of cells: Computer simulations explain cell movement

by Institute of Science and Technology Austria

Looking under the microscope, a group of cells slowly moves forward in a line, like a train on the tracks. The cells navigate through complex environments. A new approach by researchers involving the Institute of Science and Technology Austria (ISTA) now shows how they do this and how they interact with each other. The experimental observations and the following mathematical concept are published in Nature Physics .

The majority of the cells in the human body cannot move. Some specific ones, however, can go to different places. For example, in wound healing, cells move through the body to repair damaged tissue. They sometimes travel alone or in different group sizes.

Although the process is increasingly understood, little is known about how cells interact while traveling and how they collectively navigate the complex environments found in the body. An interdisciplinary team of theoretical physicists at the Institute of Science and Technology Austria (ISTA) and experimentalists from the University of Mons in Belgium now has new insights.

Much like social dynamics experiments, where understanding the interactions of a small group of people is easier than analyzing an entire society, the scientists studied the traveling behavior of a small group of cells in well-defined in vitro surroundings, i.e. outside a living organism, in a Petri dish equipped with interior features. Based on their findings, they developed a framework of interaction rules.

Cells travel in trains

David Brückner rushes back to his office to grab his laptop. "I think it's better to show some videos of our experiments," he says and presses play.

The video shows a Petri dish. Microstripes—one-dimensional lanes guiding cell movement —are printed on the substrate beside a zebrafish scale made up of numerous cells. Special wound-healing cells, known as "keratocytes" start to stretch away from the scale, forming branches into the lanes.

"At first, cells stick together through adhesive molecules on their surface—it's like they're holding hands," explains Brückner. Suddenly, the bond breaks off, and the cells assemble into tiny groups, moving forward like trains along tracks.

"The length of the train is always different. Sometimes it's two, sometimes it's ten. It depends on the initial conditions."

Eléonore Vercurysse and Sylvain Gabriele from the University of Mons in Belgium observed this phenomenon while investigating keratocytes and their wound-healing features within different geometrical patterns. To help interpret these puzzling observations, they reached out to theoretical physicists David Brückner and Edouard Hannezo at ISTA.

Cells have steering wheels

"There's a gradient within each cell that determines where the cell is going. It's called 'polarity' and it's like the cell's very own steering wheel," says Brückner. "Cells communicate their polarity to neighboring cells, allowing them to move in concert." But how they do so has remained a big puzzle in the field.

Brückner and Hannezo started brainstorming. The two scientists developed a mathematical model combining a cell's polarity, its interactions, and the geometry of its surroundings. They then transferred the framework into computer simulations , which helped them visualize different scenarios.

The first thing the scientists in Austria looked at was the speed of the cell trains. The simulation revealed that the speed of the trains is independent of their length, whether they consist of two or ten cells.

"Imagine if the first cell did all the work, dragging the others behind it; the overall performance would decrease," says Hannezo. "But that's not the case. Within the trains, all the cells are polarized in the same direction. They are aligned and in sync about their movement and smoothly move forward." In other words, the trains operate like an all-wheel drive rather than just a front-wheel drive.

As a next step, the theoreticians examined the effects of increasing the width of the lanes and the cell clusters in their simulations. Compared to cells moving in a single file, clusters were much slower. The explanation is quite simple: the more cells are clustered together, the more they bump into each other. These collisions cause them to polarize away from each other and move in opposite directions. The cells are not aligned properly, which disrupts the flow of movement and drastically influences the overall speed. This phenomenon was also observed in the Belgian lab (in vitro experiments).

Dead end? No problem for cell clusters

From an efficiency standpoint, it sounds like moving in clusters is not ideal. However, the model predicted that it also had its benefits when cells navigate through complex terrain, as they do, for instance, in the human body. To test this, the scientists added a dead end, both in the experiments and in the simulations.

"Trains of cells get to the dead end quickly, but struggle to change direction. Their polarization is well aligned, and it's very hard for them to agree on switching around," says Brückner. "Whereas in the cluster, quite a few cells are already polarized in the other direction, making the change of direction way easier."

Trains or clusters?

Naturally, the question arises: when do cells move in clusters, and when do they move in trains? The answer is that both scenarios are observed in nature. For example, some developmental processes rely on clusters of cells moving from one side to the other, while others depend on small trains of cells moving independently.

"Our model doesn't only apply to a single process. Instead, it is a broadly applicable framework showing that placing cells in an environment with geometric constraints is highly instructive, as it challenges them and allows us to decipher their interactions with each other," Hannezo adds.

A small train packed with information

Recent publications by the Hannezo group suggest that cell communication propagates in waves—an interplay between biochemical signals, physical behavior, and motion. The scientists' new model now provides a physical foundation for these cell-to-cell interactions, possibly aiding in understanding the big picture.

Based on this framework, the collaborators can delve deeper into the molecular players involved in this process. According to Brückner, the behaviors revealed by these small cell trains can help us understand large-scale movements, such as those seen in entire tissues.

In order to better understand fundamental processes, for example, in the fields of neuroscience, immunology, or genetics, the use of animals in research is indispensable. No other methods, such as in silico models, can serve as an alternative. The animals are raised, kept, and treated according to the strict regulations of the respective countries where the research is conducted.

Journal information: Nature Physics

Provided by Institute of Science and Technology Austria

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